Baculovirus Replication: Uptake of Trichoplusia ni Nuclear Polyhedrosis Virus Particles by Insect Cells
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چکیده
منابع مشابه
Baculovirus Replication: Stimulation of Thymidine Kinase and DNA Polymerase Activities in Spodopterafrugiperda Cells Infected with Trichoplusia ni Nuclear Polyhedrosis Virus
Baculoviruses are large DNA-containing viruses which replicate in the nucleus of invertebrate cells. The viruses contain supercoiled DNA of tool. wt. about 8 x l 0 7 (Bud & Kelly, 1977) and the multiply enveloped nuclear polyhedrosis virus of Trichoplusia ni used in this study has a genome size of 8.3 x 107 (Bud & Kelly, 1980). Little is known about the replication of baculoviruses in molecular...
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Nuclei isolated from Trichoplusia ni cells (TN-368) infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to study the replication of viral DNA. Based on DNA : DNA hybridization data, virus-specific DNA polymerase activity was insensitive to aphidicolin and novobiocin and was inhibited by ddTTP and N-ethylmaleimide. The data indicate that the AcMNPV-specific DNA pol...
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The insect baculovirus AcMNPV (Autographa californica multiple nuclear polyhedrosis virus) enters many mammalian cell lines, prompting its application as a general eukaryotic gene delivery agent, but the basis of entry is poorly understood. For adherent mammalian cells, we show that entry is favoured by low pH and by increasing the available cell-surface area through a transient release from th...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1985
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-66-3-541